Protoplast

Cibus Announces New Publication: Industrial Scale Gene Editing in Brassica napus

Retrieved on: 
Giovedì, Novembre 30, 2023
Gene editing, Trait, Botany, Regeneration, CBUS, Oleic acid, SAN, Protoplast, Plant, Gene, Breeding, Rapeseed, Growth, Rape, Canadian International Council, Environment, Agriculture

(Link Article: https://www.mdpi.com/2037-0164/14/4/77 )

Key Points: 
  • (Link Article: https://www.mdpi.com/2037-0164/14/4/77 )
    “The ability to scale breeding in a precise and repeatable process is the central breakthrough of our high-speed breeding process, which we refer to as the Trait Machine™ System.
  • Here, we demonstrate two case studies of improving oleic oil content and developing pod shatter reduction in Brassica napus by using gene editing tools on an industrial scale.
  • There are four BnaFAD2 genes involved in oleic oil content and eight BnaSHP genes involved in pod shatter tolerance.
  • In order to develop these two traits, we delivered nuclease ribonucleoproteins with Gene Repair Oligonucleotides (GRONs) into protoplasts, with subsequent regeneration into plants on an industrial scale, which encompassed robust tissue culture protocols, efficient gene editing, robotics sampling, and molecular screening, vigorous plant regeneration, growth, and phenotyping.