Complementary DNA

Takara Bio USA, Inc. Elevates the Sensitivity of scRNA-seq in Its Continuing Mission to Support the Efforts of Single-Cell Researcher

Retrieved on: 
Tuesday, January 26, 2021
Health, Stem cells, Genetics, Other Science, Research, Science, Biotechnology, Branches of biology, Biology, Molecular biology, Complementary DNA, Reverse transcriptase, Transcription, RNA, Single cell sequencing

We consistently find that the Takara Bio SMART-Seq Single Cell kit demonstrates greater sensitivity than our Smart-seq2 protocol.

Key Points: 
  • We consistently find that the Takara Bio SMART-Seq Single Cell kit demonstrates greater sensitivity than our Smart-seq2 protocol.
  • The SMART-Seq Single Cell Kit incorporates Takara Bios proprietary SMART (Switching Mechanism at 5 End of RNA Template) technology, which relies on the template-switching activity of reverse transcriptase to enrich for full-length cDNAs.
  • The kit was specifically created for single-cell applications and improved performance for cells with very low RNA content.
  • While there are many single-cell technologies available today, we recognize the need for higher sensitivity is especially critical when working with cells that have lower amounts of RNA.

Lexogen Introduces LUTHOR 3' mRNA-Seq Library Prep Kit for Ultra-Low Input and Single-Cell Sequencing

Retrieved on: 
Thursday, October 22, 2020
Branches of biology, Biology, Molecular biology, Biochemistry, Gene expression, Biotechnology, DNA, RNA, Transcriptomics technologies, RNA-Seq, Transcription, Complementary DNA

VIENNA, Oct. 22, 2020 /PRNewswire/ --Lexogen, a transcriptomics and Next-Generation Sequencing company today announced the introduction of the LUTHOR 3' mRNA-Seq Library Prep Kit.

Key Points: 
  • VIENNA, Oct. 22, 2020 /PRNewswire/ --Lexogen, a transcriptomics and Next-Generation Sequencing company today announced the introduction of the LUTHOR 3' mRNA-Seq Library Prep Kit.
  • LUTHOR employs a novel amplification technology called THOR (T7 High-resolution Original RNA) that amplifies RNA directly without a cDNA intermediate.
  • LUTHOR is the first kit of Lexogen's new product line for single-cell analysis based on the proprietary THOR Amplification Technology.
  • Established in 2007, Lexogen is a transcriptomics and Next-Generation Sequencing company, focusing on the development of innovative methods for RNA analysis.

Lexogen Introduces LUTHOR 3' mRNA-Seq Library Prep Kit for Ultra-Low Input and Single-Cell Sequencing

Retrieved on: 
Thursday, October 22, 2020
Branches of biology, Biology, Molecular biology, Biochemistry, Gene expression, Biotechnology, DNA, RNA, Transcriptomics technologies, RNA-Seq, Transcription, Complementary DNA

VIENNA, Oct. 22, 2020 /PRNewswire/ --Lexogen, a transcriptomics and Next-Generation Sequencing company today announced the introduction of the LUTHOR 3' mRNA-Seq Library Prep Kit.

Key Points: 
  • VIENNA, Oct. 22, 2020 /PRNewswire/ --Lexogen, a transcriptomics and Next-Generation Sequencing company today announced the introduction of the LUTHOR 3' mRNA-Seq Library Prep Kit.
  • LUTHOR employs a novel amplification technology called THOR (T7 High-resolution Original RNA) that amplifies RNA directly without a cDNA intermediate.
  • LUTHOR is the first kit of Lexogen's new product line for single-cell analysis based on the proprietary THOR Amplification Technology.
  • Established in 2007, Lexogen is a transcriptomics and Next-Generation Sequencing company, focusing on the development of innovative methods for RNA analysis.

Swift Biosciences Enables COVID-19 Surveillance with NGS of Low Titer SARS-CoV-2 Specimens

Retrieved on: 
Thursday, May 28, 2020
Natural sciences, Molecular biology, Laboratory techniques, Branches of biology, Polymerase chain reaction, Biochemistry, Reverse transcription polymerase chain reaction, Complementary DNA, Real-time polymerase chain reaction, Molecular diagnostics

Targeting the full viral genome in a single tube reaction with a rapid 2-hour workflow, Swift's assay enables research and surveillance activities by accommodating the low viral copy titers found in clinical specimens.

Key Points: 
  • Targeting the full viral genome in a single tube reaction with a rapid 2-hour workflow, Swift's assay enables research and surveillance activities by accommodating the low viral copy titers found in clinical specimens.
  • The two-round PCR kit supports high throughput processing by converting and enriching viral cDNA into multiplexed, NGS-ready libraries.
  • Following presence/absence assessment using qRT-PCR, excess cDNA was used as input into the Swift SNAP workflow to establish an NGS-based mutation profile for public health surveillance.
  • Swift Biosciences develops and commercializes NGS Library Preparation Kits that enable technologies for genomics, translational, and clinical research.

Berkeley Lights Expands Its Platform and Introduces the OptoSeq™ 3' mRNA Library Kit

Retrieved on: 
Tuesday, March 31, 2020
Branches of biology, Biology, Nucleic acids, Molecular biology, DNA, Biotechnology, Gene expression, RNA, Complementary DNA, DNA sequencing, Library, Messenger RNA

EMERYVILLE, Calif., March 31, 2020 /PRNewswire/ -- Today, Berkeley Lights, a leader in cell selection, announced their new OptoSeq 3' mRNA Library Kit enabling users to directly link phenotype to genotype.

Key Points: 
  • EMERYVILLE, Calif., March 31, 2020 /PRNewswire/ -- Today, Berkeley Lights, a leader in cell selection, announced their new OptoSeq 3' mRNA Library Kit enabling users to directly link phenotype to genotype.
  • The OptoSeq 3' mRNA Library Kit significantly expands the capabilities of the Berkeley Lights platform.
  • "With the new OptoSeq 3' mRNA Library Kit, customers can now use the Berkeley Lights platform to refine library prep, allowing users to consolidate workflows and complete the cell journey on one system."
  • The OptoSeq 3' mRNA Library Kit includes reagents, primers, probes, and enzymes to prepare and label individual cells for cDNA sequencing.

1CellBio Announces Custom Targeted Bead Program to Accelerate Next Phase in Single-Cell Analysis

Retrieved on: 
Wednesday, April 17, 2019
Health, Biotechnology, Genetics, Pharmaceutical, Research, Science, Branches of biology, Nucleic acids, Biochemistry, Gene expression, RNA, Molecular biology, Messenger RNA, RNA-Seq, Transcription, Complementary DNA, Transcriptomics technologies

1CellBio, Inc. , today announced a new program to supply select customers with its proprietary inDrop hydrogel beads synthesized with custom primers that target user-specified transcripts.

Key Points: 
  • 1CellBio, Inc. , today announced a new program to supply select customers with its proprietary inDrop hydrogel beads synthesized with custom primers that target user-specified transcripts.
  • We believe the ability to customize and target transcriptional regions of interest will accelerate clinical and pharmaceutical research applications of single-cell RNA-seq.
  • Some of the potential applications for the targeted beads might be custom oncogene panels, immunoprofiling for antibodies and T-cell receptors, or mRNA isoform detection.
  • Custom panels for targeted transcript capture are the natural next phase in single-cell analysis.

RNA sequencing transformed: Oxford Nanopore introduces new cDNA Sequencing Kits

Retrieved on: 
Monday, February 4, 2019
Technology, Data management, Software, Health, Biotechnology, Genetics, Medical devices, Oncology, Research, Other Science, Science, Branches of biology, Biology, Molecular biology, Natural sciences, Nanopore sequencing, DNA sequencing, Nanopore, Complementary DNA, Polymerase chain reaction, Sequencing, Transcriptomics technologies, Rapid amplification of cDNA ends

Oxford Nanopore has launched new 109 cDNA Kits for its real-time, scalable sequencing technology.

Key Points: 
  • Oxford Nanopore has launched new 109 cDNA Kits for its real-time, scalable sequencing technology.
  • With nanopore long read cDNA sequencing kits we obtain a much more detailed view of which transcripts are generated."
  • In combination with upgrades to the Oxford Nanopore sequencing platforms and software, nanopore cDNA sequencing delivers effective enrichment for full-length transcripts (PCR-cDNA).
  • In addition to cDNA sequencing, Oxford Nanopore also uniquely offers direct RNA analysis kits, allowing users to sequence RNA without the need to convert to cDNA.

Lexogen upgrades the TeloPrime Full-Length cDNA Amplification Kit empowering multiple downstream applications

Retrieved on: 
Monday, December 17, 2018
Molecular biology, Biology, Biochemistry, Complementary DNA, Transcription, Sequencing, Serial analysis of gene expression, Rapid amplification of cDNA ends

VIENNA, Dec. 17, 2018 /PRNewswire/ -- Today Lexogen launches a Version 2 (V2) upgrade for the TeloPrime Full-Length cDNA Amplification Kit; an all-in-one protocol for generating 5' cap-specific full-length cDNA from as little as 1 ng of total RNA.

Key Points: 
  • VIENNA, Dec. 17, 2018 /PRNewswire/ -- Today Lexogen launches a Version 2 (V2) upgrade for the TeloPrime Full-Length cDNA Amplification Kit; an all-in-one protocol for generating 5' cap-specific full-length cDNA from as little as 1 ng of total RNA.
  • The new TeloPrime V2 Kits provide higher yields and enhanced representation of longer cDNAs than the initial version.
  • Hence, methods that involve reverse transcription and sequencing of complementary DNA (cDNA) remain at the forefront of long-read transcriptome-focused studies.
  • TeloPrime-amplified cDNA therefore provides a very faithful representation of the mRNA transcriptome, empowering multiple downstream applications such as Next Generation Sequencing (NGS), single-molecule sequencing (e.g.

Lexogen upgrades the TeloPrime Full-Length cDNA Amplification Kit empowering multiple downstream applications

Retrieved on: 
Monday, December 17, 2018
Molecular biology, Biology, Biochemistry, Complementary DNA, Transcription, Sequencing, Serial analysis of gene expression, Rapid amplification of cDNA ends

VIENNA, Dec. 17, 2018 /PRNewswire/ -- Today Lexogen launches a Version 2 (V2) upgrade for the TeloPrime Full-Length cDNA Amplification Kit; an all-in-one protocol for generating 5' cap-specific full-length cDNA from as little as 1 ng of total RNA.

Key Points: 
  • VIENNA, Dec. 17, 2018 /PRNewswire/ -- Today Lexogen launches a Version 2 (V2) upgrade for the TeloPrime Full-Length cDNA Amplification Kit; an all-in-one protocol for generating 5' cap-specific full-length cDNA from as little as 1 ng of total RNA.
  • The new TeloPrime V2 Kits provide higher yields and enhanced representation of longer cDNAs than the initial version.
  • Hence, methods that involve reverse transcription and sequencing of complementary DNA (cDNA) remain at the forefront of long-read transcriptome-focused studies.
  • TeloPrime-amplified cDNA therefore provides a very faithful representation of the mRNA transcriptome, empowering multiple downstream applications such as Next Generation Sequencing (NGS), single-molecule sequencing (e.g.